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. 2019 May 26;2019:7457105. doi: 10.1155/2019/7457105

Figure 4.

Figure 4

Raddeanin A- (RA-) induced apoptosis and cell cycle arrest might occur through the regulation of the PI3K/AKT the pathway in vivo. (a,b) After treatment, the statistical analysis of tumor volume and tumor weight was conducted. The data shown are the mean ± SD (n=3, ∗P<0.05, compared with the control). (c) The necrosis of tumor tissue was detected by HE staining. Yellow arrow, inflammatory cells; black arrow, pathologic mitosis; red arrow, vacuolated cytoplasm. (d) Apoptosis cell of tumor tissue was detected by TUNEL staining. Apoptotic cells are indicated by red arrow, yellow arrow indicated normal cells (20x). (e, f, g) RT-PCR, immunoblot assay, and immunohistochemistry indicate the expression of apoptosis-related factors, cell cycle-related factors, and PI3K/AKT pathway factors. (h) HE staining tests the effect of RA on liver. β-actin was used as the internal control. The data are shown as the mean ± SD (n=3, ∗P<0.05, compared with the control).