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Effect of ASP on HCC cells-induced tube formation of HUVECs. (a) The medium from SK-Hep1 and Hep-3B cells treated previously with ASP (0, 2.5, 5, and 10 mg/ml, for 24 h) was collected and then applied to tube formation assay. (b) Representative histogram of the area covered by the tube network quantitated using Image-Pro Plus software. ∗, p < 0.05, compared with the control group.
