Table 2.
T. spiralis PSGs encoding differentially expressed proteins in adults, muscle larvae and newborn larvae stages.
| Accession No. | Description | Ratio | Ratio | Ratio |
|---|---|---|---|---|
| ML/Ad3 | ML/NBL | Ad3/NBL | ||
| E5S768/EFV59360.1 | putative chitin binding peritrophin-A domain protein | 2.26 | 0.54 | 2.89 |
|
| ||||
| E5SCG4/EFV57539.1 | angiotensin-converting enzyme, testis-specific isoform | 1.85 | 2.75 | 0.36 |
|
| ||||
| E5SG15/EFV56231.1 | putative IQ calmodulin-binding motif protein | 0.62 | 2.12 | 0.55 |
|
| ||||
| E5SJW0/EFV54917.1 | dimethylaniline monooxygenase [N-oxide-forming] 4 | - - - | 2.01 | - - - |
|
| ||||
| E5SCL8/EFV57458.1 | gamma-glutamyltranspeptidase 1 | - - - | 2.58 | - - - |
|
| ||||
| E5SHM8/EFV55721.1 | diacylglycerol O-acyltransferase 2 | 1.74 | - - - | - - - |
|
| ||||
| E5SEH3/EFV56859.1 | G2/mitotic-specific cyclin-B3 | - - - | - - - | 2.26 |
|
| ||||
| E5S186/EFV61456.1 | leucine-rich repeat and fibronectin type-III domain-containing protein 4 | - - - | 3.06 | - - - |
|
| ||||
| E5S732/EFV59418.1 | Putative fibronectin type III domain protein | - - - | 2.60 | - - - |
|
| ||||
| E5SK45/EFV54875.1 | putative cadherin domain protein, partial | - - - | 3.06 | - - - |
|
| ||||
| E5S9G6/EFV58492 | cystathionine beta-synthase | 2.14 | 0.56 | - - - |
|
| ||||
| E5S621/EFV59764.1 | mitochondrial pyruvate carrier (brain protein 44) | - - - | - - - | 1.75 |
|
| ||||
| E5S718/EFV59404.1 | putative proton-coupled amino acid transporter 4 | 2.11 | - - - | - - - |
|
| ||||
| E5RYX1/EFV62291.1 | Y+L amino acid transporter 1 | - - - | 2.51 | - - - |
|
| ||||
| E5SFC3/EFV56520.1 | excitatory amino acid transporter 1 | - - - | 2.25 | - - - |
|
| ||||
| E5S9E0/EFV58588.1 | lonCoA ligase 5 | 1.60 | 0.52 | - - - |
|
| ||||
| E5SGN7/EFV56123.1 | fatty acid synthase | 1.69 | - - - | 1.76 |
|
| ||||
| E5S673/EFV59699.1 | GDP-L-fucose synthetase | 2.20 | - - - | 1.88 |
|
| ||||
| E5RYD9/EFV62441.1 | phosphoglucomutase-2 | 2.29 | - - - | - - - |
|
| ||||
| E5SGM8/EFV56114.1 | phosphomevalonate kinase | 1.62 | - - - | 1.94 |
|
| ||||
| E5SJ10/EFV55223.1 | calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase1C | - - - | 3.93 | - - - |
|
| ||||
| E5S2V7/EFV60868.1 | conserved hypothetical protein | 1.90 | - - - | 1.93 |
|
| ||||
| E5SEI1/EFV56867.1 | conserved hypothetical protein | - - - | 2.41 | - - - |
|
| ||||
| E5S2J6/EFV60967.1 | hypothetical protein Tsp_03059 | 2.81 | 2.22 | - - - |
|
| ||||
| E5S923 /EFV58705.1 | small G protein signaling modulator 3-like protein | 1.63 | - - - | - - - |
|
| ||||
| E5SFG5/EFV56461.1 | activating signal cointegrator 1 | - - - | 2.08 | - - - |
|
| ||||
| E5SBW7/EFV57723.1 | extracellular signal-regulated kinase 1 | - - - | - - - | 1.91 |
|
| ||||
| E5SIP9/EFV55337.1 | transcriptional adapter 2-beta | 1.64 | - - - | 1.98 |
|
| ||||
| E5SIM1/ EFV55358.1 | putative BTB/POZ domain protein | 2.08 | - - - | - - - |
|
| ||||
| E5S9P2 /EFV58568.1 | putative PDZ domain protein | 1.77 | - - - | - - - |
|
| ||||
| E5SEX3/EFV56656.1 | diphthamide biosynthesis protein 1 | 1.77 | - - - | 2.10 |
|
| ||||
| E5S3A2/EFV60724.1 | zinc finger protein | - - - | 0.55 | 2.02 |
|
| ||||
| E5S8F0/EFV58915.1 | putative actin-binding protein anillin | - - - | 0.29 | 2.71 |
|
| ||||
| E5SFF4/EFV56450.1 | GTP-binding ADP-ribosylation factor | - - - | 2.44 | - - - |
|
| ||||
| E5SFH3/EFV56469 | chromatin regulator subfamily B member 1 | 1.60 | - - - | - - - |
|
| ||||
| E5SB63/EFV58014.1 | DNA topoisomerase 2-alpha | - - - | - - - | 1.78 |
|
| ||||
| E5SGN0/EFV56116.1 | histone acetyltransferase type B catalytic subunit | - - - | - - - | 1.93 |
|
| ||||
| E5S4U4/EFV60187.1 | putative thromboxane-A synthase | - - - | 2.58 | - - - |
|
| ||||
| E5SJ08/EFV55221.1 | eukaryotic translation initiation factor 3 subunit E, partial | - - - | 3.39 | - - - |
|
| ||||
| E5RZQ0/EFV61978.1 | hint module superfamily | - - - | 6.66 | - - - |
|
| ||||
| E5SWW7/EFV50703.1 | putative irregular chiasm C-roughest protein | - - - | 2.01 | - - - |
|
| ||||
| E5S3J0/EFV60653.1 | conserved hypothetical protein | 1.96 | 0.40 | - - - |
|
| ||||
| E5S3I6/EFV60649.1 | conserved hypothetical protein | - - - | 2.54 | - - - |
|
| ||||
| E5SIR3/EFV55280.1 | conserved hypothetical protein | - - - | 2.02 | - - - |
|
| ||||
| E5S3U6/EFV60533.1 | cuticle collagen 34 protein | 0.28 | 0.45 | 0.59 |
|
| ||||
| E5SC11/EFV57673.1 | Gut-specific cysteine proteinase | - - - | 0.56 | - - - |
|
| ||||
| E5S2Q2/EFV60927.1 | pyruvate dehydrogenase complex, E1 component, | |||
| pyruvate dehydrogenase, beta subunit | 0.63 | - - - | 0.58 | |
|
| ||||
| E5SET0/EFV56681.1 | bestrophin-1 | 0.55 | - - - | 0.46 |
|
| ||||
| E5SC18/EFV57649.1 | protein phosphatase PTC7-like protein | - - - | - - - | 0.61 |
|
| ||||
| E5S7E3/EFV59293.1 | phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase PTEN | - - - | 0.45 | - - - |
|
| ||||
| E5S8M1/EFV58830.1 | Phosphoribosyl pyrophosphate synthetase-associated protein 2 | 0.60 | - - - | - - - |
|
| ||||
| E5SK74/EFV54817.1 | putative BAR domain protein | 0.65 | - - - | 0.60 |
|
| ||||
| E5RZN4/EFV61962.1 | putative peptidase dimerization domain protein | - - - | 0.55 | - - - |
|
| ||||
| E5SU43/EFV51698.1 | conserved hypothetical protein | 0.45 | - - - | 0.40 |
|
| ||||
| E5S2M0/EFV60948.1 | conserved hypothetical protein, partial | 0.57 | - - - | 0.53 |
|
| ||||
| E5RYF1/EFV62453.1 | conserved domain protein | - - - | 0.46 | - - - |
|
| ||||
| E5SDL6/EFV57109.1 | conserved hypothetical protein | 0.24 | - - - | 0.19 |
Differentially expressed proteins were defined using isobaric tags for relative and absolute quantitation (iTRAQ) as those with at least a 1.5 fold change relative to one another, with p < 0.05. Ratio data shows stage specific expression of proteins from Liu et al, 2016 [21]. ML, mature larvae, Ad3, adult L3 larvae, NBL, newborn larvae.