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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Am Chem Soc. 2019 May 3;141(19):7698–7703. doi: 10.1021/jacs.9b02611

Figure 2.

Figure 2.

Generation of Dha and Dhb on proteins via intermolecular GECCO in E. coli. (a) Structure of Afb-Z complex (PDB: 1LP1) showing two proximal sites for placing FSY and the target Ser. (b-c) Tandem mass spectra identifying Ser and Dha at site 7 of the Afb protein. (d-e) Tandem mass spectra identifying FSY and Tyr at site 24 of the Z protein. (f) Structure of Afb-Z complex (PDB: 1LP1) showing two proximal sites for placing FSY and the target Thr. (g-h) Tandem mass spectra identifying Thr and Dhb at site 7 of the Afb protein. (i-j) Tandem mass spectra identifying FSY and Tyr at site 24 of the Z protein.