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Generation of Dha on sfGFP via intramolecular GECCO in E. coli. (a) Crystal structure of sfGFP (PDB: 2B3P) showing site Tyr182 for FSY incorporation to target Ser introduced at site Glu184 on the β-strand. (b-c) Tandem MS spectra of sfGFP (182FSY/184Ser) expressed in E. coli identifying 182FSY/184Ser (b) and 182Tyr/184Dha (c).
