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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Am Chem Soc. 2019 May 3;141(19):7698–7703. doi: 10.1021/jacs.9b02611

Figure 4.

Figure 4.

Generation of Dha on Afb via intramolecular GECCO in E. coli. (a-b) Tandem mass spectra identifying Dha at Ser-1 (a) and Ser10 (b) of the Afb protein. (c-d) Histogram of Cβ-Cβ distances of Ser-1 and Asp37 (c) and of Ser10 and Asp37 (d) in 4,525 low energy models of ab initio folded Afb. (e-f) Representative models from ab initio folding of Afb showing Asp37 close to Ser-1 (e) and close to Ser10 (f). The gold structure in (e) is the aligned Afb backbone of 1LP1.