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Single module extender unit competition assay. The fusion protein PikAIIITE or the final module of the DEBS PKS, Ery6TE, are incubated with the synthetic pentaketide chain mimic 10 and a mixture of the native extender 8 and an equimolar amount of 9a in vitro. NADPH was produced in situ with an NADPH regeneration system. Error was ± 5% of indicated value. Percentages are shown for wild-type systems.
