. Author manuscript; available in PMC: 2020 Feb 6.

Published in final edited form as: J Am Chem Soc. 2019 Jan 24;141(5):1961–1969. doi: 10.1021/jacs.8b10521

Table 1.

Product distributions catalyzed by domain swapped chimeras.

Enzyme	10-dML^a	Narbonolide^b		Relative Activity^c
Enzyme	3a + 4a	5a + 6a	7a	Relative Activity^c
R1	4.2%^d	23.6%^d	0.3%^d	100.0^d
R2	11.8%^d	17.9%^d	0.2%^d	9.0^d
R3	66.7%^d	37.2%^d	0.6%^d	47.5^d
R4	0.8%^d	-^f	-^f	100.0^d
R5	N.D.^e	-^f	-^f	N.D.^e
R6	7.4%^d	-^f	-^f	586.0^d
R7	7.2%^d	-^f	-^f	180.3^d
R8	N.D.^e	-^f	-^f	N.D.^e

Percent of 3a + 4a out of all 10-dML products (1, 3a, and 4a). See Scheme 1 for structures of products.

Percent of (5a + 6a) or 7a out of all narbonolide products (2, 5a, 6a, and 7a). See Scheme 1 for structures of products.

Total activity of each system relative to R1 (for bimodular systems) or R4 (for monomodular systems) is based on total amount of products for each reaction (sum of 1, 2, 3a, 4a, 5a, 6a, and 7a).

Error was ± 5% of indicated value

N.D. = Not detected

Not applicable