. Author manuscript; available in PMC: 2020 Feb 6.

Published in final edited form as: J Am Chem Soc. 2019 Jan 24;141(5):1961–1969. doi: 10.1021/jacs.8b10521

Table 2.

Product distributions catalyzed by engineered mono and bimodular Pik systems.

		Enzyme		10-dML^a	Narbonolide^b		Relative
Entry	PikAIIITE	PikAIII	PikAIV	3a + 4a	5a + 6a	7a	Activity^c
R9	WT	-^d	-^d	0.8%^e	-^d	-^d	100.0^e
R10	V753A	-^d	-^d	21.1%^e	-^d	-^d	4.2^e
R11	Y755R	-^d	-^d	N.D.^f	-^d	-^d	N.D.^f
R12	Y755V	-^d	-^d	42.2%^e	-^d	-^d	81.5^e
R13	WT (apo)	-^d	-^d	N.D.^f	-^d	-^d	N.D.^f
R14	-^d	WT	WT	4.2%^e	23.6%^e	0.3%^e	100.0^e
R15	-^d	V753A	WT	7.7%^e	21.3%^e	0.8%^e	92.5^e
R16	-^d	Y755V	WT	55.9%^e	61.9%^e	16.7%^e	82.1^e
R17	-^d	WT	Y753V	4.4%^e	77.2%^e	1.3%^e	86.8^e
R18	-^d	Y755V	Y753V	44.6%^e	59.6%^e	14.7%^e	51.7^e
R19	-^d	WT (apo)	WT (apo)	N.D.^f	N.D.^f	N.D.^f	N.D.^f

Percent of 3a + 4a out of all 10-dML products (1, 3a, and 4a). See Scheme 1 for structures of products.

Percent of (5a + 6a) or 7a out of all narbonolide products (2, 5a, 6a, and 7a). See Scheme 1 for structures of products.

Total activity of each system relative to R9 (for monomodular systems) or R14 (for bimodular systems) is based on total amount of products (sum of 1, 2, 3a, 4a, 5a, 6a, and 7a).

Not applicable

Error was ± 5% of indicated value

N.D. = Not detected