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Lycopene reporter system and preliminary characterization of IPK. (A) The reporter system module generates lycopene from cellular hemiterpenes. Fs is expected to inhibit native hemiterpene biosynthesis via the DXP pathway but not an artificial alcohol-dependent hemiterpene pathway. (B) E. coli BL21Tuner(DE3) harboring pCDFDuet-GGPP, pACYCDuet-Lyc and pETDuet-IPK in wells of a microplate were treated with combinations of IPTG, DMAA/ISO, and Fs and visualized.
