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. Author manuscript; available in PMC: 2019 Jun 9.
Published in final edited form as: Cancer Res. 2018 Mar 13;78(10):2490–2502. doi: 10.1158/0008-5472.CAN-17-2392

Figure 3. Overexpression of CaMKII in C4–2B4 cell line.

Figure 3.

C4–2B4 cells were transfected with empty vector, plasmid containing constitutively active CaMKII-T286D, wild type CaMKII, or inactive mutant CaMKII-K42M. (A) Whole cell lysates were immunoblotted for total CaMKII. Actin was used as a loading control. (B) Cells were cultured in 10% (left panel) or 1% FBS (right panel). Cell proliferation is expressed as fold change of cell number compared to day 0. n=3. (C) Cells were grown in soft agar. Number of colonies per field was counted. n=3. (D) Cells were seeded onto a Boyden chamber. Cells migrated through membrane were labeled with Calcein AM. n=2. (E) Cells were seeded into BioCoat Matrigel-coated invasion chamber. Cells invaded through the matrigel were labeled with Calcein AM. n=3. *, p<0.05.