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| Reagent | Final concentration |
|---|---|
| Tris-HCl (1M, pH 8.0) | 15mM |
| NaCl (5M) | 15mM |
| KCl (1M) | 60mM |
| EDTA (0.5M, pH 8.0) | 1mM |
| EGTA (50mM, pH 8.0) | 0.5mM |
| Spermine (500mM) | 0.5mM |
| Pefabloc SC (20mg/mL) | 0.1mg/mL |
| Dithiothreitol (1M) | 2mM |
| Protease inhibitor (Mini) | - |
| Ultra pure water | - |
Store for up to a week at 4°C. Add Pefabloc SC, dithiothreitol and protease inhibitor cocktail immediately before use.
