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| Reagent | Final concentration |
|---|---|
| Tris-HCl (1M, pH 8.0) | 10mM |
| Sucrose (1M) | 0.3M |
| MgCl2 (1M) | 5mM |
| KCl (1M) | 25mM |
| NaF (500mM) | 10mM |
| β-Glycerophosphate (1M) | 5mM |
| Sodium pyrophosphate (100mM) | 5mM |
| Spermine (500mM) | 0.5mM |
| Spermidine (500mM) | 0.5mM |
| Pefabloc SC (20mg/mL) | 0.1mg/mL |
| Dithiothreitol (1M) | 2mM |
| Protease inhibitor (Mini) | - |
| Iodixanol (60%) | 20%; 25%; 30% |
| Ultra pure water | - |
OptiPrep™ Density Gradient Medium is 60% iodixanol.
Prepare fresh and keep at 4 °C.
