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| Reagent | Final concentration |
|---|---|
| Tris-HCl (1M, pH 7.6) | 20mM |
| NaCl (5M) | 50mM |
| MgCl2 (1M) | 2mM |
| CaCl2 (1M) | 2mM |
| Pefabloc SC (20mg/mL) | 0.1mg/mL |
| Dithiothreitol (1M) | 1mM |
| Glycerol (100%) | 50% |
| Ultra pure water | - |
Aliquot to 50–100μL and store at −20 °C for up to several months
