FIG 2.

ESR1 and ESR2 gene expression. A and B, RNA-seq gene expression represented by RPKM values of ESR1 (Fig 2, A) and ESR2 (Fig 2, B) in esophageal biopsy specimens from healthy control subjects (female subjects, n = 3; male subjects, n = 3) and patients with EoE (female patients, n = 4; male patients, n = 6). ESR1 and ESR2 expression was increased in patients with EoE compared with that in control subjects. C and D, There was no difference in expression between male and female subjects in the EoE or control groups. The RPKM value was less than 1 for ESR1 and ESR2. E and F, ESR1 and ESR2 immunofluorescence staining of esophageal biopsy specimens from male and female patients with EoE and healthy control subjects: results of immunofluorescence analyses for ESR1 and ESR2 in control subjects (no eosinophils were present in the biopsy specimen) and a representative active EoE biopsy specimen (EoE; maximum of 62 [male] and 90 [female] eosinophils per high-power field) are shown. The bar is 20 μm for ×20 images (left panels) and 50 μm for ×40 images (right panels). A total of 2 control and 4 active biopsy specimens from patients with EoE were stained. The white line indicates a single-cell basal layer. The white arrow indicates a positive staining cell within the single-cell basal layer. The yellow arrow indicates positive staining within the basal proliferative zone. DAPI, 4ʹ,6-Diamidino-2-phenylindole; F, female; M, male.