TYK2 and STAT6 phosphorylation after exposure to IL-13 with or without E2 in esophageal epithelial cells. A, Representative Western blot of EPC2 lysate for phosphorylated STAT6 (P-STAT6) and STAT6 at 0, 5, 30, and 60 minutes after IL-13 (100 ng/mL) exposure with or without 24 hours of pretreatment with E2 (100 nmol/L) or vehicle (0.01% EtOH) analyzed by means of immunoblotting with anti-STAT6, anti–P-STAT6, and anti-GAPDH. Quantitative expression of P-STAT6 levels corrected to the housekeeping gene (GAPDH; n = 3). *P < .05, Student t test. B, TYK2 phosphorylation after exposure to IL-13 with or without E2 in esophageal epithelial cells. Representative Western blot of EPC2-ALI cell lysate for phosphorylated TYK2 (P-TYK2) and TYK2 at 0, 5, 30, and 60 minutes after IL-13 (100 ng/mL) exposure with or without 24 hours of pretreatment with E2 (100 nmol/L) or vehicle (0.01% EtOH). Analysis was done by means of immunoblotting with anti-TYK2, anti–P-TYK2, and anti-actin. Quantitative expression of P-TYK2 levels corrected to total TYK2 levels (n = 3). *P < .05, Student t test.