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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Dec 20;143(6):2131–2146. doi: 10.1016/j.jaci.2018.10.070

FIG 5.

FIG 5.

E2-mediated abrogation of IL-13–induced changes in esophageal barrier dysfunction is ESR2-dependent. Mature EPC2-ALI cells (>1500 Ω · cm2) were treated with either vehicle (0.1% dimethyl sulfoxide) or MPP (100 pmol/L) or PHTPP (10 μmol/L) 30 minutes before exposure to E2 (100 nmol/L). Twenty-four hours later, EPC2-ALI cells were stimulated with IL-13 (100 ng/mL) in the presence of vehicle, MPP, or PHTPP and E2 for 24 hours, and TER measurements were performed, as described in the Methods section. Data are represented as averages ± SEMs of 6 to 13 individual cultures per group. *P < .05 compared with vehicle plus IL-13 plus E2, multigroup ANOVA analyses and Holm-Sidak posttest.