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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Life Sci. 2019 Mar 16;224:187–196. doi: 10.1016/j.lfs.2019.03.035

Table 2.

Genes studied and probes used in the in vitro qRT-PCR experiments.

Target NCBI Reference sequence Forward primer Reverse primer Product length
hIL-6 NM_000600.4 5′-AAA GAG GCA CTG GCA GAA AA-3′ 5′-TTT CAC CAG GCA AGT CTC CT-3′ 99 bp
hIL-1β NM_000576.2 5′-ATG CAC CTG TAC GAT CAC TGA-3′ 5′-ACA AAG GAC ATG GAG AAC ACC-3′ 142 bp
hNF-κB (p65/RelA) NM_021975.3 5′-AAT GGC TCG TCT GTA GTG C-3′ 5′-TGC TCA ATG ATC TCC ACA TAG G-3′ 145 bp
hTNF-α NM_000594.3 5′-ACT TTG GAG TGA TCG GCC-3′ 5′-GCT TGA GGG TTT GCT ACA AC-3′ 139 bp
hα-SMA (Acta2) NM_001141945.2 5′-CTG AGC GTG GCT ATT CCT TC-3′ 5′-GCA GTG GCC ATC TCA TTT TC-3′ 109 bp
hPDGF-β receptor NM_002609.3 5′-GGC TAC ATG GAC ATG AGC AA-3′ 5′-TCG GCA GGT CCT CTC AG-3′ 150 bp
hSMAD3 (MADH3) NM_005902.3 5′-GGA GAA ATG GTG CGA GAA GG-3′ 5′-GAA GGC GAA CTC ACA CAG C-3′ 259 bp
hTGF-β1 NM_000660.6 5′-TGA ACC GGC CTT TCC TGC TTC TCA TG-3′ 5′-GCG GAA GTC AAT GTA CAG CTG CCG C-3′ 152 bp
hPPAR-γ NM_138712.3 5′-GCT GGC CTC CTT GAT GAA TA-3′ 5′-TTG GGC TCC ATA AAG TCA CC-3′ 114 bp
c-Myc NM_002467.5 5′-TCA AGA GGC GAA CAC ACA AC-3′ 5′-GGC CTT TTC ATT GTT TTC CA-3′ 110 bp
hβ-actin (ACTB) NM_001101.4 5′-GGA GAA TGG CCC AGT CCT C-3′ 5′-GGG CAC GAA GGC TCA TCA T-3′ 145 bp