Figure 2.

Knockdown of lncFOXO1 promoted cell proliferation and invasion. A549 cells were transfected with sh-FOXO1 vector. (A) The expression level of lncFOXO1 in control and sh-FOXO1 transfected cells was examined by qRT-PCR; (B) The cell viability in control and sh-FOXO1 transfected cells was detected by MTT assay; (C) The colony number in control and sh-FOXO1 transfected cells was detected by colony formation assay; (D) The invasiveness of A549 cells in control and sh-FOXO1 transfected cells was determined by Transwell assay. Data are presented as the mean ± SD of three independent experiments; * P<0.05, ** P<0.01, *** P<0.001

LncFOXO1: long non-coding RNA FOXO1; shRNA: short hairpin RNA; qRT-PCR: quantitative real time PCR; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2 5-diphenyl-2Htetrazolium bromide