Fig. 2.

Representative kinetic profiles of (A) THC depletion, (B) 11-OH-THC formation, (C) 11-OH-THC depletion by P450 enzymes, and (D) COOH-THC formation in the presence and absence of sulfaphenazole (CYP2C9 inhibitor) and itraconazole (CYP3A inhibitor) from one (of three) independent experiment, each with duplicate determinations. Inhibition studies were performed over a range of cannabinoid concentrations that spanned the nonlinear kinetic range (see Fig. 1). Substrate depletion (eq. 5) and metabolite formation (eq. 7) were used to determine kinetic parameters (V_max and K_m). Representative concentration–time curves are shown in Supplemental Fig. 2.