. 2019 Jul;47(7):743–752. doi: 10.1124/dmd.119.086470

TABLE 1.

Nonspecific, HLM incubation, and plasma protein binding of THC and 11-OH-THC

Data shown are mean ± S.D. of three independent experiments with four to six replicates per experiment.

Cannabinoid	f_adsorbed^a	fu_inc^b	fu_p^c
THC	0.927 ± 0.041	0.040 ± 0.015	0.011 ± 0.001
11-OH-THC	0.864 ± 0.049	0.061 ± 0.025	0.012 ± 0.002

^{^a}

The fraction of THC or 11-OH-THC nonspecifically bound to the LB tube surface in buffer was calculated using R; the LB surface partition ratio (eq. 1) as f_adsorbed = 1 − R/(R + 1).

^{^b}

Tube adsorption method was used to measure fraction unbound in incubations (fu_inc) of THC (500 nM) and 11-OH-THC (50 nM) in the presence of 0.2% BSA and 0.02 or 0.1 mg/ml HLM for THC and 11-OH-THC, respectively.

^{^c}

Ultracentrifugation with diluted plasma was used to measure plasma protein binding (fu_p) of THC (500 nM) and 11-OH-THC (50 nM).