Fig. 7.

GBM sensitivity to sodium sulfide is dependent on the electron transport chain. U87 and LN-18 ρ⁰ were confirmed by PCR using mitochondria specific cytochrome b primers and β-actin as a control (A). Survival of LN18 ρ⁺ and ρ⁰ following treatment with Na₂S or degassed water for 4 h was determined using clonogenic assay (B). U87 ρ⁰ cells did not form colonies. All data are from 3 independent experiments. Error bars represent SD and * represents P < 0.05 using a one-way ANOVA with Tukey's post-hoc analysis.