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. 2019 Jun 4;14:4091–4103. doi: 10.2147/IJN.S203222

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© 2019 Liu et al.

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Anticancer effect of biosynthesized gold nanoparticle from Chinese medicinal herb C. wenyujin (CWAuNPs) on apoptotic gene expression in renal carcinoma cell line A498. A498 cells were treated with 25 µg/ml and 50 µg/ml of CWAuNPs for 24 hours and after 24 hours of treatment, the control and treated cells were subjected to total RNA isolation and cDNA conversion. The cDNA of the control and treated groups were subjected to qPCR analysis with specific apoptotic genes Caspase 3, Caspase 9, Bid, Bax, and Bcl-2. Each bar represents the mean±SEM of three independent observations. P<0.05 is considered as statistically significant. *P<0.05; ^#P<0.01.