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. 2019 Jun 4;14:4091–4103. doi: 10.2147/IJN.S203222

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© 2019 Liu et al.

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Anticancer effect of biosynthesized gold nanoparticle from Chinese medicinal herb C. wenyujin (CWAuNPs) on apoptotic protein expression in renal carcinoma cell line A498. A498 cells were treated with 25 µg/ml and 50 µg/ml of CWAuNPs for 24 hours and after 24 hours of treatment, the control and treated cells were subjected to total protein isolation. The 50 µg of total protein from the control and treated groups were subjected to electrophoresis and immublotting analysis with specific apoptotic proteins Caspase 3 (Cas-3), Caspase 9 (Cas-9), Bid, Bax, Bcl2, and Bclxl. Each bar represents the mean±SEM of three independent observations. p<0.05 is considered as statistically significant. *p<0.05; ^#p<0.01.