Table 1.
Polysaccharide kinetics for BoMan26B and the variants K149S and K149A
The data include the estimation of kcat/Km for the variants K149S, K149A, W112F, and W112A using linear regression for LBG and guar gum.
| Km | kcat | kcat/Kma | |
|---|---|---|---|
| g/liter | s−1 | g/liter−1s−1 | |
| LBG | |||
| BoMan26B | 10.7 ± 1.1 | 250 ± 12.1 | 23.3 ± 2.7 |
| K149S | 21.6 ± 4.5 | 179 ± 23.1 | 8.24 ± 2.0 |
| K149A | 20.1 ± 3.7 | 178 ± 14.1 | 8.68 ± 1.7 |
| W112F | 1.2 | ||
| W112A | 0.98 | ||
| Guar gum | |||
| BoMan26B | 12.9 ± 1.7 | 122 ± 9.4 | 9.5 ± 1.4 |
| K149S | 3.2 | ||
| K149A | 3.6 | ||
| W112F | 0.71 | ||
| W112A | 0.56 |
a The kcat/Km values for classical Michaelis-Menten kinetics are in agreement with the estimated kcat/Km value based on linear regression.