Table 4.

Interactions between BoMan26B and the G1M4 ligand bound in subsites −5 to −2

Subsite	Saccharide unit	Saccharide atom	H-bond distance (including intermediate waters)	Residue atom	Interacting residue
−5	Man		Stacking		Trp-112
		O3	2.4 Å w200–2.7 Å–w173–2.9 Å	OD1	Asp-111
		O3	2.4 Å–w200–2.7 Å–w173–2.9 Å	OD2	Asp-111
		O4	2.7 Å–w173–2.9 Å	OD1	Asp-111
		O4	2.7 Å–w173–2.9 Å	OD2	Asp-111
−4a	Gal	O2	2.9 Å	NZ	Lys-149
		O3	2.6 Å	O	Tyr-148
		O4	3.1 Å	N	Tyr-148
		O4	2.9 Å	O	Tyr-148
		O6	3.1 Å–w114–2.9 Å	OD1	Asn-104
		O6	3.1 Å–w114–3.1 Å	N	Ala-107
−3	Man		Stacking		Tyr-317
−2	Man	O2	3.0 Å	NE1	Trp-314
		O2	3.1 Å–w125–3.1 Å	OE1	Glu-334
		O2	3.1 Å–w125–2.9 Å	NE2	His-335
		O6	2.8 Å	OH	Tyr-315
		O6	2.6 Å–w58–2.6 Å	O	Val-77
		O6	2.6 Å–w58–2.7 Å	OD2	Asp-101

^a Data show the interactions of the galactose side group for the mannosyl unit occupying the −4 subsite. There are no observed interactions with the enzyme for this mannosyl unit.