Table 5.

Overview of conserved GH26 residues in all sequences from the phylogenetic tree

Residues with a minimum of 97% conservation in branches A and B are shown. Characterized sequences are based on the CAZy database (www.cazy.org).⁴ Conservation within sub-branches other than A and B is shown in parentheses. Residues in the active-site cleft are marked in bold, with the catalytic residues (E201 and E291) underlined (50). These residues are in the following subsites D101 (−2), H136 (−1), R197 (−1), H200 (−1), G204 (+1), W206 (+1), F207 (−1), D261 (+1), Y263, (−1) and W314 (−1).

* Residues were previously stated to be conserved in GH26 β-mannanases (33, 44).