Figure 3.

S. Typhimurium–encoded CspE increases the stability of yciF mRNA. The yciF mRNA stability was determined in terms of the relative amounts of its transcript levels. A, relative mRNA levels of yciF were determined upon bile treatment in the WT/VA strain, by Northern blotting and quantitation. B, mRNA levels of yciF were determined with or without transcription inhibition by rifampicin. The mRNA levels were quantitated relative to the amounts at 0-min post-addition of rifampicin, in the yciF overexpressing strains (WT/pyciF and ΔcspE/pyciF), using 500 μg/ml rifampicin, added at the end of the 8th of growth. Data are presented as mean ± S.E. and are representative of three independent experiments. *, p < 0.05; **, p < 0.01.