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. 2019 Apr 16;294(23):9084–9099. doi: 10.1074/jbc.RA119.008209

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© 2019 Ray et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — S. Typhimurium–encoded CspE binds to yciF mRNA, and the Phe-30 residue is essential for this role. A, thermal stability curve of purified StCspE and StCspE^F30V proteins obtained using CD spectroscopy at 219-nm wavelength. B, qualitative and quantitative analysis of in vivo stability of 2× FLAG-tagged StCspE and StCspE^F30V obtained using Western blotting with antibody against the FLAG tag. C, binding of StCSpE and StCspE^F30V to full-length yciF mRNA. Lane 1, mRNA alone; lanes 2–6, increasing concentration of StCspE; lane 7, StCspE^F30V. The filled triangle represents increasing concentrations of purified protein. D, graphical representation of complex formation by StCspE, as a function of protein concentration. Data are presented as mean ± S.E. and representative of three independent experiments.