Figure 7.

ΔcspE exhibits increased permeability upon bile salts stress, which is rescued upon cspE complementation, but not by the F30V mutant of CspE. A, representative fluorescence image 45 min after addition of bisBenzimide H 33258 in WT and ΔcspE, without (0%) or with (3%) pretreatment with bile salts for 5 h. B, quantification of bisBenzimide H 33258 accumulation in WT/VA-, ΔcspE/VA-, cspE-complemented strains (WT/pcspE and ΔcspE/pcspE) and cspE-F30V-complemented strains (WT/pcspE-F30V and ΔcspE/pcspE-F30V), and yciF overexpressing strains (WT/pyciF and ΔcspE/pyciF) without (0%) or with (3%) pretreatment with bile salts for 5 h. Data are presented as mean ± S.E. and representative of three independent experiments.