Table 1.

Overexpression of yciF suppresses the bile sensitivity of ΔcspE

Quantification of kinetic growth determined in terms of log₁₀ (CFU/ml) values of WT and ΔcspE with the following vectors: VA, pcspE, pcspE-F30V, and pyciF grown with and without 3% bile salts. Values are represented as mean ± S.E. Statistical analysis was performed using two-way ANOVA, and the different comparisons have been represented using the following symbols: #, p < 0.001. Comparison of each strain treated with 3% bile salts with respect to WT/VA grown in 3% bile salts is shown. a, p < 0.0001; b, p < 0.01. Comparison of each strain grown in 3% bile salts with respect to ΔcspE/VA grown in 3% bile salts is shown.

Time	WT/VA	WT/pcspE	WT/pcspE-F30V	WT/pyciF	ΔcspE/VA	ΔcspE/pcspE	ΔcspE/pcspE-F30V	ΔcspE/pyciF
h
0% bile salts
0	5.72 ± 0.59	5.52 ± 0.48	6.25 ± 0.07	5.51 ± 0.53	5.83 ± 0.28	5.54 ± 0.11	6.35 ± 0.07	5.33 ± 0.29
6	9.35 ± 0.18	9.48 ± 0.17	8.68 ± 0.38	9.42 ± 0.13	9.65 ± 0.11	9.57 ± 0.08	8.63 ± 0.22	9.51 ± 0.06
12	11.14 ± 0.44	11.48 ± 0.15	10.55 ± 0.13	10.72 ± 0.15	10.58 ± 0.02	11.36 ± 0.42	10.47 ± 0.16	10.81 ± 0.42
3% bile salts
0	5.69 ± 0.64	4.59 ± 0.15	6.47 ± 0.12	5.1 ± 0.52	4.06 ± 0.82	5.15 ± 0.44	4.01 ± 0.33	5.73 ± 0.23
6	8.52 ± 0.23	8.80 ± 0.26	8.37 ± 0.18	7.79 ± 0.51	4.59 ± 0.73#	8.50 ± 0.414a	5.1 ± 0.34#	7.61 ± 0.2b
12	10.55 ± 0.24	11 ± 0.21	10.56 ± 0.31	10.04 ± 0.5	5.71 ± 0.34#	10.71 ± 0.72a	5.68 ± 0.47#	11.31 ± 0.26a