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. 2019 May 6;294(23):9225–9238. doi: 10.1074/jbc.RA119.008915

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© 2019 Kornilov et al.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Figure 3. — Purification and high-resolution MS of lipids generated by oxidation of arachidonate to isolate the synthetic lipid. A, semipreparative normal-phase HPLC as indicated in under “Experimental procedures” with collected fractions labeled A–D. The HPLC was monitored online at 206 nm. B, further HPLC purification of Peak B (Fig. 1A) as indicated under “Experimental procedures.” The HPLC was monitored online at 206 nm. C, high-resolution (Orbitrap) LC-MS/MS of platelet lipid (top) versus Peak B (bottom) as indicated under “Experimental procedures.” mAU, milli-absorbance units.