Figure 6.

¹H NMR data of the synthetic lipid continued. A, region of epoxy protons ¹H NMR (benzene-d₆:methanol-d₄, 90:10; 700 MHz) δ 3.04–3.01 (dt, 1H, J = 2.20, 5.1 Hz), 2.84–2.83 (dd, 1H, J = 2.13, 3.83 Hz), 2.66–2.64 (dt, 1H, J = 2.25, 5.20 Hz), 2.62–2.60 (dt, 1H, J = 2.21, 5.36 Hz). B, 2D COSY. C, structure of the synthetic generated diEHEDE isomer (±)8,9-trans-11(R),12(R)-trans-DiEp-13(R)-HEDE. D, structure of the proposed platelet lipid 8,9–11,12-DiEp-13-HEDE. Shown here is the isomer proposed based on studies using mutant COX-2 isoforms, 8(R),9(R)-cis-11(R),12(R)-trans-diEp-13(R)-HEDE.