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. 2019 Apr 25;294(23):9134–9146. doi: 10.1074/jbc.RA118.006000

Figure 6.

Figure 6.

Specific C-C chemokine signaling pathways were likely important barriers in cardiac reprogramming that were overcome by IMAP treatment. A and C, bar graph or scatter plots showing normalized -fold changes of α-MHC–GFP+ cells 2 weeks after GMT infection with indicated chemical treatment in (A) MEFs and (C) NCFs. B and D, bar graph representing major cardiac and fibroblast genes as determined by qPCR in cells after 2 weeks of reprogramming and indicated chemical treatment in (B) MEFs and (D) NCFs. E and F, representative images of immunofluorescence staining (E) and quantification of (F) cardiac markers α-MHC–GFP (green), cTnT (red), and double positive cells in NCFs (control) or cells treated with either MGT+DMSO or MGT+3i. G, bar graph showing normalized -fold changes of α-MHC–GFP+ cells 2 weeks after GMT infection with indicated C-C chemokine ligands treatment in MEFs (scale bar, 500 μm). H, bar graph showing normalized -fold changes of α-MHC–GFP+ cells 2 weeks after GMT infection with indicated treatment in MEFs. I, schematic diagram showing IMAP-enhanced cardiac reprogramming by suppressing the expression of several specific C-C chemokine ligands and receptors. Error bars indicate mean ± S.E.; #, p < 0.05; ##, p < 0.01; ###, p < 0.001; ####, p < 0.0001 compared with MGT+3i group; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 compared with MGT+DMSO group.