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. 2019 Apr 29;294(23):9186–9197. doi: 10.1074/jbc.RA118.007122

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© 2019 Duan et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 7. — Histone methylation represses ULK1 and ATG16L1 expression. A, MHM and SJSA1 were treated 24 h with vehicle or Nutlin (10 μm) in the presence or absence of DZnep (10 μm). mRNA for ULK1 and ATG16L1 were determined and presented as graphs with S.D. indicated (triplicate). The asterisk indicates there are significant differences between vehicle and Nutlin treatment in control MHM and SJSA1 cells (p < 0.05), and between Nutlin-treated and Nutlin plus DZnep-treated cells (p < 0.01). B, whole cell lysates were immunoblotted for the indicated proteins. The double asterisk indicates there are significant differences between Nutlin treated and Nultin plus DZnep treated cells (p < 0.01).