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Kinetic analysis of SaOhyA. A, the apparent Km for FAD calculated using 20 μm 18:1(9Z) substrate. The line represents the fit of the data points to the Michaelis–Menten equation. B, the apparent 18:1(9Z) Km determined at 50 μM FAD substrate. The line represents the fit of the data points to the Hill equation. The dependence of the reaction on 18:1(9Z) was highly cooperative with a Hill number of 2.2 ± 0.3. Error bars, S.E.
