Table 1.

Unsaturated fatty acids as substrates of SaOhyA

SaOhyA assays were performed as described under “Experimental procedures” using LC–MS to detect hydroxy-fatty acid formation and identify the location of the hydroxyl group (see Fig. 4).

Fatty acid	Product
16:1(6Z)	Not detected
16:1(9Z)	10-Hydroxy-16:0 (h16:0)
16:1(9E)	Not detected
18:1(9Z)	10-Hydroxy-18:0 (h18:0)
18:1(9E)	Not detected
18:1(11Z)	Not detected
18:2(9Z,12Z)	10-Hydroxy-18:1 (12Z) (h18:1)
18:3(9Z,12Z,15Z)	10-Hydroxy-18:2 (12Z,15Z)
18:3(6Z,9Z,12Z)	10-Hydroxy-18:2 (6Z,12Z)
20:4(5Z,8Z,11Z,14Z)	Not detected
22:6(4Z,7Z,10Z,13Z,16Z,19Z)	Not detected