Table 1.
Apoptotic fractions in TNBC cell lines exposed to homoharringtonine
| HHT (ng/ml) | AF | T0 | T6 | T24 | ||||||
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| 0 | 0 | 20 | 50 | 100 | 0 | 20 | 50 | 100 | ||
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| MDA-MB-157 | ||||||||||
| Early | 9.1 ± 4.2 | 8.9 ± 4.0 | 14.7 ± 3.0 | 21.8 ± 3.1 | 23.7 ± 4.5 | 13.6 ± 2.1 | 36.2 ± 3.3 | 38.6 ± 2.8 | 48.2 ± 3.5 | |
| Late | 4.7 ± 2.2 | 5.9 ± 1.6 | 7.2 ± 1.9 | 8.2 ± 0.6 | 12.6 ± 1.8 | 5.5 ± 1.3 | 12.7 ± 1.5 | 17.3 ± 3.3 | 21.1 ± 1.6 | |
| Total | 13.7 ± 4.7 | 14.8 ± 3.7 | 21.9 ± 1.6 | 30.0 ± 3.2 | 36.3 ± 2.7 | 19.1 ± 3.3 | 48.9 ± 4.8 | 55.9 ± 0.5 | 69.3 ± 4.1 | |
| MDA-MB-468 | ||||||||||
| Early | 5.2 ± 1.7 | 7.4 ± 1.2 | 8.3 ± 1.4 | 12.1 ± 4.1 | 14.7 ± 4.5 | 7.2 ± 0.8 | 9.5 ± 1.3 | 14.4 ± 4.9 | 18.1 ± 5.5 | |
| Late | 2.7 ± 1.2 | 4.4 ± 1.1 | 3.8 ± 0.9 | 3.8 ± 0.5 | 4.6 ± 0.9 | 2.7 ± 0.9 | 3.5 ± 1.7 | 4.5 ± 0.7 | 6.2 ± 0.6 | |
| Total | 7.9 ± 2.8 | 11.8 ± 1.7 | 12.1 ± 2.3 | 15.9 ± 4.2 | 19.2 ± 3.8 | 9.9 ± 1.5 | 13.0 ± 2.8 | 19.0 ± 5.4 | 24.3 ± 5.7 | |
| MDA-MB-231 | ||||||||||
| Early | 2.7 ± 0.5 | 2.2 ± 0.5 | 2.8 ± 1.2 | 3.0 ± 0.7 | 4.2 ± 1.7 | 2.6 ± 0.7 | 3.4 ± 1.0 | 6.0 ± 1.6 | 4.6 ± 1.3 | |
| Late | 2.4 ± 1.0 | 2.8 ± 1.0 | 2.7 ± 0.5 | 2.9 ± 0.8 | 3.5 ± 1.1 | 2.8 ± 0.8 | 2.3 ± 0.2 | 5.6 ± 2.3 | 4.2 ± 1.8 | |
| Total | 5.1 ± 1.4 | 5.0 ± 1.4 | 5.5 ± 1.7 | 6.0 ± 0.9 | 7.7 ± 2.1 | 5.5 ± 1.2 | 5.7 ± 1.0 | 11.6 ± 3.6 | 8.8 ± 1.8 | |
| CAL-51 | ||||||||||
| Early | 4.2 ± 1.0 | 7.4 ± 1.6 | 7.2 ± 1.8 | 7.8 ± 2.0 | 9.1 ± 2.8 | 5.3 ± 1.5 | 11.6 ± 2.8 | 16.9 ± 0.7 | 15.5 ± 1.3 | |
| Late | 4.4 ± 2.0 | 1.9 ± 2.2 | 3.8 ± 0.9 | 3.9 ± 0.9 | 5.4 ± 1.2 | 3.7 ± 1.2 | 3.6 ± 0.1 | 5.1 ± 0.6 | 6.1 ± 1.0 | |
| Total | 8.6 ± 1.1 | 9.3 ± 0.7 | 11.0 ± 2.6 | 11.7 ± 2.6 | 14.5 ± 2.8 | 9.0 ± 1.2 | 15.2 ± 2.8 | 22.0 ± 0.2 | 21.6 ± 1.1 | |
|
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| HHT (ng/ml) | AF | T48 | T72 | |||||||
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|
|
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| 0 | 20 | 50 | 100 | 0 | 20 | 50 | 100 | |||
|
| ||||||||||
| MDA-MB-157 | ||||||||||
| Early | 14.6 ± 3.8 | 39.4 ± 2.7 | 48.8 ± 2.8 | 53.9 ± 4.2 | 9.9 ± 2.7 | 45.5 ± 3.9 | 53.7 ± 4.6 | 56.3 ± 3.0 | ||
| Late | 5.1 ± 1.3 | 15.2 ± 1.9 | 19.7 ± 0.6 | 20.0 ± 4.6 | 6.4 ± 2.5 | 17.5 ± 2.8 | 22.0 ± 4.6 | 26.1 ± 1.2 | ||
| Total | 19.7 ± 3.7 | 54.6 ± 3.9 | 68.5 ± 2.8 | 73.9 ± 4.6 | 16.3 ± 4.7 | 62.9 ± 1.1 | 75.7 ± 4.0 | 82.4 ± 2.5 | ||
| MDA-MB-468 | ||||||||||
| Early | 8.3 ± 1.2 | 14.5 ± 3.3 | 17.6 ± 4.1 | 21.9 ± 5.8 | 8.7 ± 1.2 | 17.1 ± 4.1 | 23.6 ± 5.5 | 27.5 ± 4.5 | ||
| Late | 3.8 ± 1.3 | 6.3 ± 0.2 | 6.4 ± 0.9 | 6.3 ± 1.5 | 3.6 ± 0.5 | 6.0 ± 0.2 | 4.6 ± 1.8 | 5.0 ± 1.3 | ||
| Total | 12.1 ± 1.7 | 20.7 ± 3.5 | 24.0 ± 3.2 | 28.3 ± 6.0 | 12.3 ± 0.8 | 23.1 ± 4.1 | 28.2 ± 6.1 | 32.5 ± 5.7 | ||
| MDA-MB-231 | ||||||||||
| Early | 2.2 ± 0.9 | 3.5 ± 1.7 | 5.4 ± 1.4 | 5.8 ± 1.3 | 2.3 ± 1.0 | 3.2 ± 2.1 | 4.4 ± 2.2 | 4.1 ± 1.3 | ||
| Late | 1.5 ± 0.4 | 3.7 ± 2.2 | 4.4 ± 1.0 | 4.2 ± 1.8 | 2.4 ± 1.2 | 2.8 ± 1.0 | 2.9 ± 0.7 | 3.2 ± 0.2 | ||
| Total | 3.7 ± 0.5 | 7.2 ± 3.1 | 9.8 ± 1.5 | 10.0 ± 0.5 | 4.7 ± 1.7 | 6.0 ± 2.8 | 7.3 ± 2.9 | 7.4 ± 1.4 | ||
| CAL-51 | ||||||||||
| Early | 5.5 ± 0.8 | 16.0 ± 2.8 | 24.4 ± 2.7 | 35.6 ± 4.2 | 4.7 ± 1.4 | 25.0 ± 2.9 | 33.6 ± 4.4 | 37.6 ± 4.0 | ||
| Late | 3.9 ± 0.3 | 5.8 ± 0.5 | 6.9 ± 0.4 | 8.6 ± 1.1 | 4.3 ± 1.7 | 8.4 ± 0.9 | 8.5 ± 1.4 | 8.4 ± 1.2 | ||
| Total | 9.4 ± 0.7 | 21.8 ± 2.6 | 31.3 ± 3.1 | 44.2 ± 3.5 | 9.1 ± 2.5 | 33.5 ± 2.1 | 42.1 ± 2.9 | 46.0 ± 3.4 | ||
AF, apoptotic fraction; HHT, homoharringtonine; T0, start of the experiment; T6, after 6 h of exposure; T24, after 24 h of exposure; T48, after 48 h of exposure; T72, after 72 h of exposure. All values are given in percentages (%) and represent mean ± SD of three independent experiments.