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. 2019 May 1;9(5):927–944.

Figure 4.

Figure 4

MiR-873 inhibits NDFIP1 expression by targeting its 3’-UTR. A. Mature miR-873 sequences and recognition sites within 3’-UTR of NDFIP1. The seed sequence of miR-873 is shown. WT and mutant NDFIP1-3’-UTR targets are also shown. B. Constructs of Renilla luciferase [Luc; (pRL)-CMV] containing WT or mutant NDFIP1 3’-UTR. C. Luciferase activity of pRL and modified constructs containing WT or mutant 3’-UTR. Luciferase constructs were cotransfected with pre-miR-Control (50 nM) or pre-miR-873 (50 nM) into HEK293T cells. Renilla luciferase activity was measured and normalized to firefly luciferase. D. Immunoblot of endogenous NDFIP1 expression in HEK293T cells 4 days after transfection of increasing amounts of pre-miR-873. E. The correlation between miR-873 and NDFIP1 mRNA expression in 20 human HCC samples using quantitative PCR. F. Spearman’s correlation was analyzed. qRT-PCR data of NDFIP1 expression in 20 paired HCC and adjacent non-tumor tissues. G. Representative IHC relationship between NDFIP1 protein expression and miR-873 are shown. Scale bar 10 μm. *P < 0.05 and **P < 0.01.