Figure 2.

Up-regulation of SSH3 promoted the invasion and metastasis of CRC cell in vitro. A. Overexpression of SSH3 in SW480 and SW620 cells analyzed through Western blot. β-tubulin was used as a loading control. B. The overexpression of SSH3 lead to a marked increase in the number of protrusions in SW480 and SW620 cells in three-dimensional morphogenesis assays. The bar chart represents the filopodia/cell. Error bars represent the means ± SD calculated from three parallel experiments. C. Overexpression of SSH3 increased cell migration as determined by transwell assays. The bar chart represents the migration cell numbers. Error bars represent the means ± SD of 5 different fields. D. Wound healing assay used to determine the migration ability of cells with up-regulated SSH3.