Figure 6.

SSH3 is involved in the cytoskeleton signaling pathway by interacting with LIMK1 and Rac1. A. Colocalization of SSH3 and LIMK1 in CRC cells. B. Extracts of HT29 cells were subjected to Co-IP using SSH3 antibody or control IgG, and Western blot was performed with LIMK1 and Rac1 antibodies. Reciprocal Co-IPs were performed using LIMK1, Rac1 and IgG antibodies, followed by Western blot with SSH3, Rac1 or LIMK1 antibodies. C. Expression of LIMK1, p-LIMK1, Cofilin and p-Cofilin when SSH3 was up-regulated or down-regulated using Western blot. GAPDH was using as a loading control.