Table 4.
Effect of CRISPR interference of the down-regulated genes on growth and production of pinene in E. coli BW25113(PT5-dxs).
| Gene | Protein | Ratio of growtha | Ratio of pinene concentrationb |
|---|---|---|---|
| Lipid metabolism | |||
| ydiJ | putative FAD-linked oxidoreductase | 1.07 ± 0.01 | 1.06 ± 0.02 |
| Metabolism of cofactors and vitamins | |||
| yjbQ | thiamine phosphate synthase | 1.08 ± 0.02 | 1.08 ± 0.02 |
| Genetic Information Processing | |||
| prpR | propionate catabolism operon regulatory protein | 1.06 ± 0.01 | 1.07 ± 0.01 |
| marR | transcriptional repressor of multiple antibiotic resistance | 1.22 ± 0.02 | 1.09 ± 0.01 |
| fabR | transcriptional repressor of fabA and fabB | 1.09 ± 0.01 | 1.07 ± 0.02 |
| Cellular Processes | |||
| cedA | cell division modulator/catalase HPII, heme d-containing | 1.07 ± 0.01 | 1.07 ± 0.02 |
| ydiJ-yjbQ-prpR-marR-fabR-cedA | 1.26 ± 0.02 | 1.13 ± 0.02 | |
a
The ratio of OD600 with CRISPRi and without.
b
The ratio of pinene production with CRISPRi and without.-: means no change or negative effect.