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. 2019 May 15;11(5):2784–2800.

Figure 6.

Figure 6

Altered FAT4 levels regulated the Hippo signalling pathway and the PCR-array analysis of HEC-1B-NC and HEC-1B-shFAT4 including 70 common ubiquitin and deubiquitin enzymes. A: The mRNA expression of the core modulators of the Hippo pathway (LATS1, LATS2, MST1, MST2, TAZ and YAP) in HEC-1A and HEC-1B cells infected with either NC or shFAT4 and in RL95-2 cells infected with NC or sgRNA, The data are presented as the mean ± SD, t test. *P<0.05, **P<0.01. B: LATS1, P-LATS1, LATS2, P-LATS2, YAP and P-YAP protein expression in HEC-1A and HEC-1B cells infected with either NC or shFAT4 and in RL95-2 cells infected with NC or sgRNA, The data are presented as the mean ± SD, t test. *P<0.05, **P<0.01. C: FAT4 repression increased the nuclear accumulation of YAP. Cytoplasmic P-YAP expression decreased, nuclear YAP levels increased, and cytoplasmic total YAP levels remained stable in FAT4-silenced cells compared with NC cells (HEC-1A and HEC-1B). GAPDH and histone 3 were used as loading controls. The data are presented as the mean ± SD, t test. *P<0.05, **P<0.01. D, E: Cluster analysis and volcano plot of the difference genes regulated by FAT4, red point represents upregualted genes and green point downregulated genes; F: Validating the significantly regulated genes (USP45, USP46, USP51, TRAF2, RNF19A, IRAK1 and RNF181) by FAT4 in HEC-1A and HEC-1B infected with either NC or shFAT4. The data are presented as the mean ± SD, t test. *P<0.05, **P<0.01.