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. 2019 May 15;11(5):3069–3080.

Figure 3.

Figure 3

Ectopic expression of FOXO1 induces arrest of G0/G1-to-S transition in PDAC cell lines. (A, B) Cell cycle assay showed that overexpression of FOXO1 induces arrest of G0/G1-to-S transition BxPC3 (A) and PANC1 (B) cell lines. (C, D) mRNA levels of CCND1 and CCNE1 in FOXO1-overexpressing BxPC3 (C) and PANC1 (D) stable cell lines. (E) CCND1 expression is negatively correlated with FOXO1 expression in fresh PDAC tissues. (F) Protein levels of CCND1 in FOXO1-overexpressing BxPC3 and PANC1 cell lines. (G) The CCND1 mRNA level is negatively correlated with FOXO1 expression in fresh PDAC tissues. (H) mRNA level of MYC in FOXO1-overexpressing BxPC3 and PANC1 stable cell lines. (I, J) Dual luciferase reporter assays revealed that overexpression of FOXO1 inhibits Wnt/β-catenin signaling activity (TCF/β-catenin reporter) in BxPC3 (I) and PANC1 (J) cell lines, but not in cells carrying mutated TCF/β-catenin reporter plasmid. (K) Overexpression of FOXO1 impairs nuclear translocation of β-catenin in BxPC3 and PANC1 cell lines. (L) Immunofluorescence assays revealed that overexpression of FOXO1 impairs nuclear β-catenin translocation nuclear in BxPC3 cells. **P < 0.01. All assays were conducted in triplicate.