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. 2019 May 15;11(5):2801–2815.

Figure 5.

Figure 5

RMRP is directly targeting to miR-613. A. miRcode algorithm was used to predict the binding site of RMRP on miR-613. RMRP sequence (ACATTCC) had a complementary binding site for miR-613 (UGUAAGG). B. Diagram of the constructed RMRP reporter plasmid containing the wild-type (WT) and mutant (MUT) binding site for miR-613. C. The WT and MUT pGL3-RMRP reporter plasmids were co-transfected into Hep3B and HCCLM3 cells with miR-613 mimic or mimic control, and luciferase activities were measured by dual luciferase reporter gene assay. miR-613 mimic inhibited the luciferase activity in Hep3B and HCCLM3 cells transfected with WT pGL3-RMRP reporter plasmid. D. miR-613 expression was measured by qRT-PCR assay in Hep3B and HCCLM3 cells after transfection with siRNA-RMRP and siRNA-control. The experiments were performed in triplicate. *P < 0.05 and **P < 0.01.