Figure 6.

RMRP sponges miR-613 to promote cell proliferation, migration, and invasion in HCC. A. qRT-PCR assay was performed to measure the miR-613 expression in Hep3B and HCCLM3 cells treated with siRNA-RMRP and miR-613 inhibitor. The promotive effect of RMRP knockdown on miR-613 in HCC cells was reversed by miR-613 inhibitor. B. RMRP silence inhibited the growth of Hep3B cells, while addition of miR-613 inhibitor reversed this effect. C. The migration of HCCLM3 cells following transfection with siRNA-RMRP and miR-613 inhibitor. D. Proportion of cells in various phases of the cell cycle in Hep3B cells. Hep3B cells transfected with siRNA-RMRP and miR-613 inhibitor decreased an accumulation of cells in the G0/G1 phase, and this decrease was accompanied with a concomitant increase of cell number in S phase. E. Cotransfected with siRNA-RMRP and miR-613 inhibitor converted HCCLM3 cells with higher capabilities of cell-cycle G1/S transition. F. Migratory ability (Magnification, × 200 times) of Hep3B and HCCLM3 was suppressed by siRNA-RMRP, and the effect was attenuated by miR-613 inhibitor. G. siRNA-RMRP negatively affected cell invasion (Magnification, × 200 times), and the suppressive effect siRNA-RMRP presented was reversed by miR-613 inhibitor but not by inhibitor control in Hep3B and HCCLM3 cells, individually. Mean of three independent experiments with SD were shown. *P < 0.05 and **P < 0.01.