Figure 5.

Transdifferentiation of co-cultured ADSCs into SGALCs. ADSCs co-cultured without SGCs were negative controls and SGCs were positive controls. (A) Morphological changes in ADSCs were observed after co-culture. IF staining confirmed the expression of (B) α-amylase and (C) AQP-5 in co-cultured ADSCs. Green indicates the protein of interest and blue indicates nuclear staining with DAPI. (D) The expression of α-amylase and AQP-5 after different amounts of time in the co-culture system was evaluated by western blotting. (E) The relative mRNA levels of α-amylase and AQP-5 increased with amount of time in the co-culture system. Scale bar = 100 μm.