Table 1.

Sequences of primers used to detect species‐specific pathogenic gene(s) in Escherichia coli, Salmonella spp., Yersinia spp. and Staphylococcus spp

Organism	Target Gene	Primer Sequence	Annealing Temp (°C)	Amplicon (bp)	Reference
E. coli	hly	F: ACGATGTGGTTTATTCTG GA	58	165	DesRosiers et al. 2001;
		R:CTTCACGTGACCATACAT AT
	stx1	F:ACACTG GATGATCTCAGTGG	58	614	DesRosiers et al. 2001;
		R:CTGAATCCCCCTCCATTATG
	stx2	F:CCATGACAACGGACAGCAGTT	58	779	Islam et al. 2015;
		R:CCTGTCAACTGAGCAGCACTTT
	eae	F:CCCGAATTCGGCACAAGCAT	59	881	Oswald 2000;
		R:CCCGGATCCGTCTCGCCAGTA
Salmonella spp.	–	ST11:AGCCAACCATTGCTAAATTGGCGCA	60	429	Aabo et al. 1993;
		ST15:GGTAGAAATTCCCAGCGGGTACTG
Yersinia spp.	pla	F:ATCTTACTTTCCGTGAGAAG	55	478	Hinnebusch & Schwan 1993;
		R:CTTGGATGTTGAGCTTCCTA
	ail	F:TAATGTGTACGCTGCGAG	57	351	Thoerner et al. 2003;
		R:GACGTCTTACTTGCACTG
Staphylococcus spp.	fnbA	F:GCGGAGATCAAAGACAA	50	1279	Signas et al. 1989
		R:CCATCTATAGCTGTGTGG

Temp, temperature; °C, degree centigrade; bp, base pair; F, Forward primer; R, Reverse primer.