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. Author manuscript; available in PMC: 2019 Nov 13.
Published in final edited form as: Nat Neurosci. 2019 May 13;22(6):897–908. doi: 10.1038/s41593-019-0399-y

Fig. 5: Differential chromatin landscapes induced by Ascl1 and Neurog2 shape the binding patterns of the shared downstream TFs.

Fig. 5:

a, tSNE plots showing the cells that express downstream TFs (Top cluster iA, below cluster iN – Fig. 1e). The dots are colored by the expression levels of downstream TF Brn2, Ebf2, and Onecut2 (n=1 cell differentiation). b-d, ChIP-seq heatmaps of endogenous Brn2 (A), Ebf2 (B), and Onecut2 (C) binding in iA and iN neurons at 48h after induction of Ascl1 and Neurog2. “iA>iN” designates sites enriched in iA neurons, “iN>iA” designates sites enriched in iN neurons, and “iA=iN” designates shared binding in both neurons (n=2). e, Metagene plots of accessibility (ATAC-seq reads) overlap at the differentially bound sites of Brn2 (left), Ebf2 (middle), and Onecut2 (right) in iA neurons (iA>iN sites).f, Metagene plots of accessibility (ATAC-seq reads) overlap at the shared sites of Brn2 (left), Ebf2 (middle), and Onecut2 (right) in iA and iN neurons (iA=iN sites). g, Metagene plots of accessibility (ATAC-seq reads) overlap at the differentially bound sites of Brn2 (left), Ebf2 (middle), and Onecut2 (right) in iN neurons (iN>iA sites).