Fig. 4.

SND1 upregulates RhoA transcription by inducing long-range chromatin conformation remodeling on the RhoA promoter. (A) SND1-binding sites predicted in the RhoA promoter (top figure) and ChIP-qPCR analyses of the capacities for them to bind with SND1. (B) Binding between SND1 and the RhoA promoter verified by EMSA (Lanes 2, 4 and 5). (C and D) 3C results showed the cross-linking efficiency between SND1 binding sites R1 and R2 in both U118MG and primary GBM cells with or without SND1 knockdown (R1 as the anchor, C; R2 as the anchor, D). DpnII digestion sites are labeled by the vertical lines, and the arrows represent the individual PCR primers. **P < 0.01. (E) Schematic displaying the assembling process of the transcription activation complex on the RhoA promoter.